Characterizing a standardized BioPart for BAG-specific expression in C. elegans

by Xinglin Qian, Sarah AlHarbi, Christian Frøkjær-Jensen
Year: 2024 DOI: 10.17912/micropub.biology.001150

Extra Information

MicroPubl Biol. 2024; 2024: 10.17912/micropub.biology.001150.
Published online 2024 Mar 12. 
PMCID: PMC10966388
PMID: 38545436

Abstract

Biological parts (BioParts) are modular and standardized DNA sequences that encode biological functions and contribute to the efficient biological engineering of complex systems. Here, we characterize a short BioPart (P flp-17 , 300 bp) for bright multicopy and single-copy BAG-specific expression starting from the gastrula stage in hermaphrodite and male C. elegans . We have generated standardized P flp-17 cloning vectors for BAG-specific gfp and mScarlet expression compatible with extra-chromosomal arrays and for single-copy transgene insertion. The short P flp-17 promoter is easy to generate by gene synthesis and has been incorporated into our online transgene design tool (www.wormbuilder.org/transgenebuilder ) .